ABSTRACT
The present study was attempted to investigate whether polyphenolic compounds isolated from wine, which is brewed from Rubus coreanum Miquel (PCRC), may affect the release of catecholamines (CA) from the isolated perfused adrenal medulla of the spontaneously hypertensive rats (SHRs), and to establish its mechanism of action. PCRC (20~180 microgram/ml) perfused into an adrenal vein for 90 min relatively dose-dependently inhibited the CA secretory responses to ACh (5.32 mM), high K+ (56 mM), DMPP (100 micrometer) and McN-A-343 (100 micrometer). PCRC itself did not affect basal CA secretion (data not shown). Also, in the presence of PCRC (60 microgram/ml), the CA secretory responses to veratridine (a selective Na+ channel activator (10 micrometer), Bay-K-8644 (a L-type dihydropyridine Ca2+ channel activator, 10 micrometer), and cyclopiazonic acid (a cytoplasmic Ca2+ -ATPase inhibitor, 10 micrometer) were significantly reduced, respectively. In the simultaneous presence of PCRC (60 microgram/ml) and L-NAME (an inhibitor of NO synthase, 30 micrometer), the inhibitory responses of PCRC on the CA secretion evoked by ACh, high K+, DMPP, and Bay-K-8644 were considerably recovered to the extent of the corresponding control secretion compared with that of PCRC-treatment alone. The level of NO released from adrenal medulla after the treatment of PCRC (60 microgram/ml) was greatly elevated compared with the corresponding basal level. Taken together, these results demonstrate that PCRC inhibits the CA secretion from the isolated perfused adrenal medulla of the SHRs evoked by stimulation of cholinergic receptors as well as by direct membrane-depolarization. It seems that this inhibitory effect of PCRC is mediated by blocking the influx of calcium and sodium into the adrenal medullary chromaffin cells of the SHRs as well as by inhibition of Ca2+ release from the cytoplasmic calcium store at least partly through the increased NO production due to the activation of NO synthase.
Subject(s)
(4-(m-Chlorophenylcarbamoyloxy)-2-butynyl)trimethylammonium Chloride , 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester , Adrenal Medulla , Calcium , Catecholamines , Chromaffin Cells , Cytoplasm , Dihydropyridines , Dimethylphenylpiperazinium Iodide , Indoles , NG-Nitroarginine Methyl Ester , Nitric Oxide , Nitric Oxide Synthase , Polyphenols , Rats, Inbred SHR , Receptors, Cholinergic , Sodium , Veins , Veratridine , WineABSTRACT
PURPOSE: The purpose of this study was to explore changes in the test items in the preparation book for the national qualifying examination. Test items which developed in the year 2000 and 2004 through nationwide faculty workshop in Fundamentals of Nursing were examined. METHOD: Test items for the national qualifying examination for 2000 and 2004 in Fundamentals of Nursing were analyzed using frequencies and percent. RESULTS: The test items for the national qualifying examination for 2004 in Fundamentals of Nursing showed an increase in the number of test item over the year 2000. According to McGuire's taxonomy, domains of the test items in 2000 were recall(65.1%), interpretation (22.0%), and problem solving(12.9%) in that order. In 2004 items for the recall domain(74.7%) increased 9.6% above the level of 2000, and interpretation and problem solving domains showed a slight decrease. With regard to type of test items, the proportion of A type in 2004 was the same with 37.6% as 2000, and that of K type(61.5%) increased by 0.7% over the year 2000(60.8%). CONCLUSION: The test items for the national qualifying examination of 2000 and 2004 were not different except for the increase in the number of test items.